At 11:12 1999-03-26 , you wrote:
>In article <7delfn$141 at net.bio.net>, radomski <radomski at unixg.ubc.ca>
>writes
>>Has anyone come across an article that deals with the overall accuracy of
>>sequencing PCR products? I just replied that the error is
>>probably the same as the error rate of the Taq used in the PCR.
>
The error will most probably not show up in a "normal" sequencing run!
Worst case scenario:
You have 1 (one) DNA copy in your PCR...
An error is introduced in the first run....
You end up with a 1:4 ratio for that base...
Normally you have problems to distinguish
heterocygozity below 1:10
/Anders
***********************************************
Anders Holmberg
Royal Institute of Technology (KTH)
Dep of Biochemistry
100 44 Stockholm, Sweden
Phone : +46 8 790 82 87
Fax : +46 8 24 54 52
Email : cato at biochem.kth.se
Web : www.biochem.kth.se
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