Does anyone have any knowledge concerning the maximum
amount of fluorescense an ABI 377 can detect and still
maintain a linear relationship between the mass of
fluorophore labelled DNA and fluorescense intensity?
In my experience, it seems to be somewhere around 4100
(rfu) but this is only empirical.
Thanks!
Mark
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Mark E. Berres 4-360 Genetics
Department of Genetics 445 Henry Mall
Department of Zoology Madison, WI. 53706-1313
University of Wisconsin at Madison Ph: (608)-262-5578