IUBio

72 lanes on the 373/377

Harold G. Hills hhills at iastate.edu
Mon Jun 28 10:09:54 EST 1999


In response to Michelle Obenauer.


Michelle,

We run 80 lanes on the 377 using combs from the Gel Co in the 96 lane mode.
You do have to do a significant amount of manual tracking particularly when
you have failed lanes which happens constantly in a core situation.  When
operating in the 96 lane mode, it is looking for a narrower lane and
therefore has trouble tracking weak or blank lanes.  We load manually.  The
spacing on the 80 lane combs is the same as the spacing on the 64 lane.

Hal
Harold G. Hills, Ph.D. DNA Sequencing Specialist	515 294-9585
1184 Molecular Biology Building			    FAX 515 294-1597
Iowa State University                                   hhills at iastate.edu
Ames, IA 50011-3260
http://www.biotech.iastate.edu/Facilities/DSSF/ABRF   For ABRF 97 DNA
sequencing tutorial.
http://www.biotech.iastate.edu/Facilities/DSSF   For info about facility.




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