Any problems using Phred/Phrap with the ABI3700 chromat files? I
tried Phred out on some DNA standard sequences (*.ab1 files) from the
3700 and the histogram of high quality bases gave very low results.
(Less than 200 bases for all 96 capillaries--even though most of the
data was pretty good.)
Does anyone have any advice?
Phillip San Miguel
Purdue Genomics Facillity