IUBio

ABI 3700 and Phred/Phrap

Phillip San Miguel pmiguel at purdue.edu
Thu Jun 3 17:06:23 EST 1999


    Any problems using Phred/Phrap with the ABI3700 chromat files?  I
tried Phred out on some DNA standard sequences (*.ab1 files) from the
3700 and the histogram of high quality bases gave very low results.
(Less than 200 bases for all 96 capillaries--even though most of the
data was pretty good.)
    Does anyone have any advice?
Phillip San Miguel
Purdue Genomics Facillity




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