Richard Cook had a question about the 80 lane combs.
Dick,
They are 80 well combs from the Gel Company. We load them by hand partly
because we do custom sequencing; that is we may use 4 or 5 cyclers for a
bunch of reactions to accomodate primer conditions etc. Not that much
longer than doing the 64 wells. The well size is the same as 64 well comb.
It does add a problem because the 96 well upgrade looks for a narrower lane
than the 80 lane comb. Requires more manual tracking when there are lanes
that fail.
The gel company also makes a 96 well comb that is 0.2mm thick to be used
with the standard plates. Bruce Roe's group uses them and loads with a
multichannel loader.
The Gel Company
665 Third Street #240
San Francisco, CA 94107
415 247-8760
CAK80U-020 is the part number for 80 well
CAK96C-020 is for 96 Custom Silk
CAK96U-020 is for 96 Sharkstooth
The custom silk provides a larger well.
Harold G. Hills, Ph.D. DNA Sequencing Specialist 515 294-9585
1184 Molecular Biology Building FAX 515 294-1597
Iowa State University hhills at iastate.edu
Ames, IA 50011-3260
http://www.biotech.iastate.edu/Facilities/DSSF/ABRF For ABRF 97 DNA
sequencing tutorial.
http://www.biotech.iastate.edu/Facilities/DSSF For info about facility.
