Storage of sequencing reactions

Bernd Weisshaar weisshaa at mpiz-koeln.mpg.de
Wed Feb 10 21:17:48 EST 1999

Dear Anja,

We had one BD kit (5000 reactions) which did become really bad over time in
the freezer. The latest results with that kit were significantly decreased
C signals, and there was some sort of a "n+1" problem (I can not explain
the cause of that). We had aliquoted the kit, and after that the aliquots
were only thown for use. After we detected the problem, it was reproduced
for that single kit also by PE/Abi. We got replacement (I can not complain
about their service!).

I should state clearly that this happened only to one single kit - and we
consume a numer of these 5000-reaction kits per year. But the problem was
storage-time dependent (about 6 to 8 months), and we store our kits prior
and after aliquotting at -20 degrees. However, one should note that there
are a number of parameters which interfere with the stability of reagents
(constant cooling, contamination during aliquotting, bad tubes or pipette
tips, etc.).

Best regards, Bernd

>I have the impression that big dye sequencing reactions that have been
>stored at -20 are not as good as the "fresh" ones. Does anybody have an ide=
>how to improve the quality of stored reactions. We do either ethanol
>precipitation or G50 columns to purify the reactions and they are dried
>before storage.
>Bye Anja
>Dr. Anja Penger
>Inst. of Plant Biology
>University of Zurich
>Zollikerstr. 107
>8008 Zurich
>Phone	Lab: 	++41 1 634 82 28
>Fax : 		++41 1 634 82 57
>e-mail:		apenger at botinst.unizh.ch


PD Dr. Bernd Weisshaar
MPI fuer Zuechtungsforschung
Carl-von-Linn=E9-Weg 10
50829 Koeln

Tel. office: +49-221-5062 590   Tel. lab: +49-221-5062 311
=46ax: +49-221-5062 313
e-mail: weisshaa at mpiz-koeln.mpg.de
WWW-URL: http://www.mpiz-koeln.mpg.de/~weisshaa/BW-info.html

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