Shaker question

Stephen R. Lasky srlasky at cellworks.mbt.washington.edu
Mon Dec 6 13:41:43 EST 1999

Julie McCollum wrote:
We use serveral approaches for solving this problem:

1) get "Tuperware" containers that hold 4 deep well plates arranged in
one layer, cover that with a sterile filter paper (whatman 3mm), cover
that with a piece of packing foam (the grey stuff), and then put that in
a normal orbital shaker incubator and attach to the platten with
springs.  This holds the plates in place and you can rotate as fast as
you want providing you don't splash the media out of the wells.

2) buy a hygro-incubator from genemachines.  This machine that allows
you to pump O2 into your incubation chambers and shake the plates hard. 
It is a self contained incubator/orbital shaker and can hold  16 deep
well plates at a time.  We don't use the O2 feature (clogging problems
and lack of need) and have had breakdowns quite often.  

The tuperware solution is low tech ahd has been used in our labs for at
least 3 years.  It works.  The hygro is new and has some problems.

hope that helps.

Stephen R. Lasky, Ph.D.			#
University of Washington		#
Department of Molecular Biotechnology	#
HSB K324 box 357730			#
Seattle, WA, 98195 USA			#
email:	srlasky at u.washington.edu	#
Phone: 	206-616-5865			#
Fax:	206-685-7301			#

> Howdy!
> We have a brand new 3700 (been running now for all of a month) and
> we're triying to scale everything up to match the capacity of the
> sequencer  (we had been running all our sequencing on a 377).  So far,
> everything's been working decently, except for our cell growth
> situation.
> How do you all grow your cells (prior to plasmid preps) in deep-well
> plates?  Specifically, what kind of shaker/incubator do you use?  Right
> now, we are using a "shaker" (it shakes more like a vortexer) which we
> stuck in a 37 deg. constant temp. room.  This shaker was bought when we
> were doing BAC preps in 96-well format, and was supossedly designed to
> hold 96-well plates.  Our problem is that to get good enough aeration,
> we have to turn this shaker up pretty high, but if you do that, the
> plates jump around and eventually end up flinging themselves off the
> shaker and onto the floor (upside down, of course).  Obviously not a
> good thing.  We have been taping the plates to the shaker, but that's a
> royal pain, and I'm tired of it.
> Does anyone have any advice?  Any special shaker we HAVE to have that
> will make my life easier?
> Thanks!
> Julie
> ===========================================
> Julie McCollum (julie at algodon.tamu.edu)
> College Station, TX

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