Barbara Simionati wrote:
> [...]
> I woul like to try to re-use membrane combs for more than one run
> otherwise they would be too expensive.
> Does anyone have tryed it already?
> [...]
George Grills (of Albert Einstein College) gave a presentation at the ABRF
session at the Genome Sequencing and Analysis Conference this year. He said
they had reused porous paper combs with no difficulty. They had checked to
see if any sequence products remained in the combs after electrophoretic
loading. They found no trace. Also, they had stored loaded combs for some
time at 4 oC and -20 oC and these had produced good sequence.
I tried reusing a porous paper comb once and got some blue blobs which I
thought might be a breakdown product of Blue Dextran. (The Blue Dextran does
not all migrate from the comb during electrophoretic loading.) But maybe the
blobs were from something unrelated.
Phillip San Miguel
Purdue Genomics Center
