Dear fellow sequencers,
We're wondering if it's time replace our glass plates (377 standard,
36cm.). They've been used constantly for over 3 years. We are plagued
occasionally by mobility problems in some lanes and the "disappearing -
reappearing" bands. We have temporarily solved the problem by "acid/base"
washing the plates. We have used Multiterge, Citrinox, Alconox, and
Seque-soap to wash the plates, but the problem inevitably returns. (We
understand a very hot DI water rinse works well, but we can't afford a lab
dishwasher).
My question is, does age "predispose" plates to this problem or do proper
cleaning methods allow plates to be used indefinately?
We are considering buying plates from Sigma. They're half the price of
ABI's and their ad claims use by genome centers and core facilities
worldwide! Are they comparable to ABI's?
The latest issue of American Biotechnology Laboratory has an ad for a
product called GELPLATE-clean. It claims that this product removes the
contaminanting molecules on the glass surface without the need for soaking.
Does anyone have experience with this product? Does it leave any
fluorescent residue?
Any advice is welcomed.
Best regards,
Skip Vaught
DNA Sequencing Service
University of Arizona
THE DNA SEQUENCING SERVICE
UNIVERSITY OF ARIZONA
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Skip Vaught- jev at u.arizona.edu
Brian Coullahan- coullaha at u.arizona.edu
Mark Schwartz- schwartm at u.arizona.edu
Amy Raymond- raymonda at u.arizona.edu