ABI sequencing kits

Elizabeth Smith ems217 at psu.edu
Thu Apr 1 07:03:45 EST 1999

I am doing DNA sequencingn on a chip using an in-house built system with
an Ar ion laser.  I am currently using the ABI BigDye Primer sequencing
kit and am not seeing any fluorescence from these dyes. ABI tells me the
excitation wavelengths for these dyes are proprietary, so I don't know
if I'm using the correct wavelength!  I was wondering if anyone has used
this ABI kit with an Ar ion laser or if you could suggest a better kit.
Liz Smith

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