Hello,
I am doing DNA sequencingn on a chip using an in-house built system with
an Ar ion laser. I am currently using the ABI BigDye Primer sequencing
kit and am not seeing any fluorescence from these dyes. ABI tells me the
excitation wavelengths for these dyes are proprietary, so I don't know
if I'm using the correct wavelength! I was wondering if anyone has used
this ABI kit with an Ar ion laser or if you could suggest a better kit.
Thanks,
Liz Smith
