Sequening bacs with big dye chemistry?

Mairead Casey mcasey at mail.med.cornell.edu
Sat May 2 16:01:58 EST 1998

I am looking for information on sequencing bac's with regard to the new
'big dye' chemistry of the ABI Prism 377.  How do you prepare your
template, cycle sequence reaction, etc.  I have been unsuccessful in my
attempts with stardard ABI protocols with regard to template and primer

Any susgestions welcome.

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