Li-COR sequencers

P. R.Laud prlaud at hal-pc.org
Tue Mar 31 08:44:08 EST 1998

	Does anyone have any tips for sequencing PCR products (~500 bp long)
using Li-COR's automated 4000 sequencer? I'm using samples someone else
prepared about 7-8 months ago on a 25-cm gel (0.25 mm thichness) and all
I get are blobs running down the length of the gel. How long can the
termination reactions last till they start to go bad? Thanks for any
input on this,

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