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200-300 dip in DNA sequences

Harold G. Hills hhills at iastate.edu
Fri Mar 27 04:31:59 EST 1998


There are several users who have found that taurine buffer solves this dip.
It is a lot simpler to just change buffer and move forward.  Since we have
used taurine buffer the problem has never returned.  We wash our plates in
alconox, rinse with very hot water and then Deionized water.  None of the 3
washes with this and two of that etc.  No special detergents.

In addition the taurine is a 20X conc. so has to be made less often, never
preciptates and goes into solution immediately instead of the 20-30 minutes
to get the TBE components to dissolve.

Now, I have found one person who tried taurine and did not get improvement.

Hal

Harold G. Hills, Ph.D. DNA Sequencing Specialist	515 294-9585
1184 Molecular Biology Building			    FAX 515 294-1597
Iowa State University                                   hhills at iastate.edu
Ames, IA 50011-3260
http://www.biotech.iastate.edu/Facilities/DSSF/ABRF   For ABRF 97 DNA
sequencing tutorial.
http://www.biotech.iastate.edu/Facilities/DSSF   For info about facility.





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