250-300 dip

Harold G. Hills hhills at iastate.edu
Fri Mar 20 17:23:33 EST 1998

Note to Emma Puttick.

Could not mail you directly.  Check out the ABRF tutorial at URL below for
the item under Taurine buffer.

>We have been experiencing a consistent loss of peak height intensity and
>resolution in 30bases in the 250-300bp region. We see this on every gel
>and in every sample. After that region the intensity and resolution
>improves and we can usually read out to 600-700bp. We have seven 377's,
>which we run 2-3 times/day doing 4 hour runs with 29:1 gels and we see
>it on every gel and it doesn't matter which chemistry we are running.
>This week we will be doing a series of experiments, on the reverse side
>of the plates, which will hopefully isolate the reagent(s) which are
>causing this problem of build-up on our plates. Our second problem is
>restoring the original side of the plates.
>I have heard that MultiTerge is meant to fix this problem, but we cannot
>buy it in Australia, yet.
>Does anyone have any ideas on what is causing this effect and how to
>eliminate it? Are there alternatives to MulitTerge?
>Any sugeestions are very much appreciated!
>Emma Puttick
>DNA Sequencing Supervisor

Harold G. Hills, Ph.D. DNA Sequencing Specialist	515 294-9585
1184 Molecular Biology Building			    FAX 515 294-1597
Iowa State University                                   hhills at iastate.edu
Ames, IA 50011-3260
http://www.biotech.iastate.edu/Facilities/DSSF/ABRF   For ABRF 97 DNA
sequencing tutorial.
http://www.biotech.iastate.edu/Facilities/DSSF   For info about facility.

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