Inverted repeats

David.Fruhling at cadus.com David.Fruhling at cadus.com
Fri Jun 12 14:01:01 EST 1998

I am having trouble sequencing through a region of inverted repeats.  I
have tried building new primers and raising the temperature of my primer
annealing and extension steps.  None have worked.  I still get 150 very
strong bases and then the sequence drops off dramatically.  Any hints,
chemicals I can add to the reactions, etc.

Thank you,
David S. Fruhling

david.fruhling at cadus.com
Cadus Pharmaceuticals

More information about the Autoseq mailing list

Send comments to us at biosci-help [At] net.bio.net