In article <6amvi8$641 at net.bio.net> Michael Tan, mstan at tally.UCHSC.edu
writes:
>Hello,
>>just want to know what types of configs everyone is using. I would like
>to compile a list and evaluate the topologies..... e.g. which computers
>are mostly used....sequencers...software etc....
>and make it available for sequencing core managers, or others
>interested--to provide info on network system design and implementation
>with high throughput DNA sequencing technology.
>
Seems like a good idea, but how about adding some basic information about
the
sort of sequencing that goes on in your facility? For example, we mostly
do
small-scale high-accuracy sequencing of plasmid constructs (up to 20kb)
before
generating transgenic animals, using custom primers and terminators (just
started
with Big Dyes). Two full gels/week.
We have
1 377, standard, with a 7200/90, 48MB, System 7.6 with collection 2.1,
Analysis
2.1.2, Factura 1.2 or6, and AutoAssembler 1.4
1 PowerTower 166, 64MB, system 7.5.3, and
1 6500/250, 64MB, OS 8
both with Factura1.2or6 and AutoAssembler 1.4.
Typically, rxns are cycled on a MJR PTC200, run on the 377 48cm wtr,
retracked etc
on the 7200, and clipped and assembled on any of the 3 macs. We also use
BLAST
and the Lasergene software.
Anthony.
