For about 20 months since owning our ABI377 Sequencer we have successfully
been able to keep our glass plates more or less free of 'stray green
fluorescence blobs' by doing a post sequence run wash of Alconox/tap
water/distilled water/air dry. From about November 1997 we have had a more
serious problem and have up until now been able to wash out the 'blobs' by
doing very long preruns or starting a run. Now we appear only to be able to
solve it by alkali/acid washing the plates after every run. Somehow after
each run a fluorescent residue remains firmly attached to the plates. Does
anyone else have a similar history of experience and/or can recommend a
quick, simple, safe remedy?
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Jane Bygraves
Division of Bacteriology
NIBSC
Blanche Lane
Potters Bar
HERTS
(01707) 654753 (x444)