dilution buffer for BigDyes

Harold G. Hills hhills at iastate.edu
Wed Feb 25 20:18:23 EST 1998

I have just been talking with our PE-ABD sales rep who says that yes, the
5X dilution buffer should be diluted in half and used at 2.5X to replace
the DB premix.

When using 1 ul of the BigDye premix, the results are clearly better with
2.5X than with 5X.

Check this out.


Harold G. Hills, Ph.D. DNA Sequencing Specialist	515 294-9585
1184 Molecular Biology Building			    FAX 515 294-1597
Iowa State University                                   hhills at iastate.edu
Ames, IA 50011-3260
http://www.biotech.iastate.edu/Facilities/DSSF/ABRF   For ABRF 97 DNA
sequencing tutorial.
http://www.biotech.iastate.edu/Facilities/DSSF   For info about facility.

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