3 hr run with 700 bases called

Mark Mucha mucham at genome.es.dupont.com
Wed Feb 4 21:58:41 EST 1998

I am wondering if any labs have altered the ABI 377XL running parameters
for their acrylamide gels to yield a read length of 650 -700 bases with
a trim length of 450-550 bases in a 3 hr run.  If so, I would like the
information.  I am currently running for 3.5 hrs to obtain these results
on 4.5% premixed Sooner gels.

Mark Mucha

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