377 gel well problem

WZhao68 wzhao68 at aol.com
Wed Feb 4 12:48:35 EST 1998

Hello there, I am using 4% Acrylamide gel on 377 (18g Urea, 5 ml of 40%
acrylamide stock, 5 ml of 10x TBE for 50 ml total volume). All of sudden, the
gel well was getting more and more unflat as usual. Even worse, the two plates
were getting tight and I could not insert the shark teeth comb in for loading.
Any suggestions?
Thanks a lot

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