Hi Muhammad,
Did you use a 0.4 mm 96 lane comb with 0.2 mm spacers or 0.4 mm spacers for
your 48-lane gels? Was this on a 377 or a 373S?
- George
At 08:30 AM 8/18/98 -0700, you wrote:
> 8/18/98
> 8:23 AM
>> RE:96 Well upgrade
>Hal,
>>We tried using regular plates, the ones that we use for our 48-lane gels,
with 96-well comb and got good results. We tried it in a desparate attempt
to solve leakiness of combs. We have done any more tests and comparisons
w.r.t. resolution but initial gels looked fine.
>By the way it did not solve our problem with the leaky combs.
>>Best regards
>Muhammad A. Lodhi
>Axys Pharmaceuticals, Inc. La Jolla, CA
>>>___________________________________________________________
George Grills
Director
DNA Sequencing and Oligonucleotide Facilities
Albert Einstein College of Medicine
713 Ullmann Building
1300 Morris Park Avenue
Bronx, New York 10461-1602
Tel: (718) 430-2657
Fax: (718) 430-8778
E-mail: grills at aecom.yu.edu
DNA Sequencing: http://leper1.ca.aecom.yu.edu/dnacore
Oligonucleotide: http://sequence.aecom.yu.edu/oligo
___________________________________________________________
