IUBio

highthoughput plant DNA

Bernd Weisshaar weisshaa at mpiz-koeln.mpg.de
Tue Aug 18 17:17:44 EST 1998


>Does anyone know of a highthroughput plant DNA purification system?  We
>have a biorobot and Qiagen doesn't make a system yet.  I tried the normal
>cell prep after we ground down the leaves but we got almost nothing.  Does
>anyone have a protocol that can be adapted to highthroughput or know of a
>kit that can do it.


Dear Tom,

we are also very much interested in such a system. I do not know a company
offering a system for DNA isolation from plant material. We grind by hand
(liquid N2) and use the Amersham Kit for A. thaliana. Any additional
suggestion welcome.

Regards, Bernd

_____________________________________________________________________

PD Dr. Bernd Weisshaar
MPI für Züchtungsforschung
Carl-von-Linné-Weg 10
50829 Köln
Germany

Tel. office: +49-221-5062 590   Tel. lab: +49-221-5062 311
Fax: +49-221-5062 313
e-mail: weisshaa at mpiz-koeln.mpg.de
WWW-URL: http://www.mpiz-koeln.mpg.de/~weisshaa/BW-info.html





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