Hello,
I am interested to do cycle sequencing of plasmid DNA with the BigDye
Terminator kit. Does anyone have a good cycling protocol to use with our
MJ Research PTC-200 PCR machine?
Many thanks for your attention!
J.E. Mejia
Institute of Molecular Medicine
John Radcliffe Hospital
Oxford OX3 9DS, UK
jmejia at worf.molbiol.ox.ac.uk