BigDye and MJ Research cycler

Jose E. Mejia jmejia at worf.molbiol.ox.ac.uk
Tue Apr 21 16:35:40 EST 1998


I am interested to do cycle sequencing of plasmid DNA with the BigDye
Terminator kit. Does anyone have a good cycling protocol to use with our
MJ Research PTC-200 PCR machine?

Many thanks for your attention!

J.E. Mejia

Institute of Molecular Medicine
John Radcliffe Hospital
Oxford OX3 9DS, UK
jmejia at worf.molbiol.ox.ac.uk

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