IUBio

AbiPrism 377 sequencing problems

John John
Tue Sep 9 08:31:04 EST 1997


In article <5u1aon$1e9 at net.bio.net>, Daniel.Marc, marc at tours.inra.fr says:
>
>Jens Dieterich ("dieteric", at paju.oulu.fi) wrote:
>: The samples seem not to run properly in the gel any more. Lanes might
>: cross during the run, parts seem to be compressed others are stretched 
>: in the same lane in the same gel. Basecalling becomes very inconvenient
>: this way and even the different extractions don't give reliable results.
>

Is the CCD detector moving properly?
If something happens to that, the software may interpret that in rather 
odd gel
images. We have seen similar problems, cured by a reset.

John Fox

******************************************************************
Alta BioScience                         Email: altabios at bham.ac.uk
School of Biochemistry                  Phone: 0121-414-5450
The University of Birmingham            Fax:   0121-414-3376
Edgbaston, BIRMINGHAM, B15 2TT, UK



More information about the Autoseq mailing list

Send comments to us at biosci-help [At] net.bio.net