In my experience, when making TBE,it is important to thoroughly dissolve
the Tris and EDTA before adding the Boric acid. If the Tris and EDTA are
stirred for 15-20 minutes before adding in the Boric acid, it will almost
never precipitate.
A much easier solution is to switch to Tris Taurine buffer made as a 20X
stock that never precipitates, does not need to be autoclaved and because
it is a 20x stock you make it half as often. Remember to add 1/2 amount to
your gels because it is 20 x and not 10 x. At the ABRF URL below there is
a recipe in the Taurine buffer html. Thank Laura Livingstone of UNC for
working this out.
Hal
Harold G. Hills, Ph.D. DNA Sequencing Specialist 515 294-9585
1184 Molecular Biology Building FAX 515 294-1597
Iowa State University hhills at iastate.edu
Ames, IA 50011-3260
http://www.biotech.iastate.edu/Facilities/DSSF/ABRF For ABRF 97 DNA
sequencing tutorial.
http://www.biotech.iastate.edu/Facilities/DSSF For info about facility.
