In reply to:
To: autoseq at net.bio.net
Date: Wed, 12 Nov 1997 09:07:17 +1100 (EST)
From: Michelle Gleeson <michelle at molecule.bio.uts.edu.au>
Subject: LiCors and PAGE PLUS Acrylamide?
Hi,
I just saw a post mentioning use of Amresco`s PAGE PLUS acrylamide as an
alternative to Long Ranger, on ALF machines. Has anyone out there tried
this on a LiCor 4000 series (66cm plates)?
AATAGGCAATGGGCCCCATATAGGAACACAGAGCTGCATGCGTATTGCATGCCAGGCTATTCATTCCAGGGAA
A
Michelle Gleeson
Molecular Parasitology Unit Ph: (02) 9514 4043
University of Technology Fax:(02) 9514 4003
Westbourne St Gore Hill, NSW 2065 michelle.gleeson at uts.edu.au
When you get to the end of your rope, tie a knot and hang on - FDR
TTATCCGTTACCCGGGGTATATCCTTGTGTCTCGACGTACGCATAACGTACGGTCCGATAAGTAAGGTCCCTT
T
Michelle,
We typically run Page Plus Acyrylamide here at LI-COR on our 66 cm gels
(in addition to Long Ranger). We generally run a 3.75% gel
concentration
with a 1.2x TBE in the gel and a 1x TBE running buffer at the standard
electrophoresis parameters. Typically this will give us 1000-1400 bp
reads, comparable to the Long Ranger, so if you wanted to try it you
should be able to get some nice data. Feel free to contact us at
biohelp at bio.licor.com if you have any other questions.
Jeff Harford
Product Specialist
LI-COR Biotech
4308 Progressive Avenue
Lincoln, NE 68504
800-645-4267
402-467-0700
jharford at bio.licor.com
