I have sent a plasmid to be sequenced using a Perkin Elmer automated
system. They use the ABI PRISM dye terminator cycle sequencing kit.
I can PCR the sequence out (with low efficiency), but am unable to get
any sequence using the components above. The plasmid and ORF isn't GC
rich and my primers are desalted/deprotected. Any suggestions would be
helpful. Thanks in advance.
