IUBio

Resolution Loss after 150 bp

tucker_a at umbsky.cc.umb.edu tucker_a at umbsky.cc.umb.edu
Wed May 21 12:08:04 EST 1997


Dear Alan

I had the same problem a couple of weeks ago. It was due to a build up of
dried acrylamide on the plates.  It's important to wash the plates just 
after
a run is completed to avoid this.  I switched to new plates and the 
problem 
disappeared.  I soaked the old plates and spacers in NaOH overnight and 
rinsed
with HCL to removed the junk.

Amy



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