IUBio

Resolution Loss after 150 bp

Alan Alan
Wed May 21 08:19:47 EST 1997


Dear Sequencers,

We are suffering a loss of resolution on some of our gels from about 
150 bp on.  On Genescan gels these means markers are not visible after 
150 bp and on sequencing gels basecalling is not good.  The problem is 
intermittent but regular.  It seems that our usual sudden loss of 
resolution at about 550 bp has just moved down the gel.

It has been suggested that it may be a build up junk on some plates.  
Does anybody know?  Has anybody had the problem and been able to fix 
it?  Is our usual loss of resolution at 550 bp normal or is that 
avoidable too?

Thanks

Alan Wilton
a.wilton at unsw.edu.au



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