AgnesTay wrote:
: > We are using an ABI 373A sequencer and for the last two runs,
only
: >150 bases of sequences were collected for every sample. The gel image
: >looks fine - all the bands can be clearly seen - but is "shortened"
such
: >that when you scroll to the top, you can see sharp clear bands typical
of
: >those for less than 200 bases of sequences.
: >
: > There is no error message and according to the log, the run
took
: >the full 14 hours. Perkin-Elmer suggested that we optimise the desktop
: >and we have done that (in fact we do that every month) to no avail.
: >
: > Please advise.
This happened to me, turned out that the votls had dropped for some
reason, doesn't sound like that here, though. Have you checked that it
isn't
a problem with the sample preps?
Ang
