IUBio

Truncated sequences

Agnes Agnes
Mon Mar 24 19:57:18 EST 1997


>        We are using an ABI 373A sequencer and for the last two runs, only
>150 bases of sequences were collected for every sample. The gel image
>looks fine - all the bands can be clearly seen - but is "shortened" such
>that when you scroll to the top, you can see sharp clear bands typical of
>those for less than 200 bases of sequences.
>
>        There is no error message and according to the log, the run took
>the full 14 hours. Perkin-Elmer suggested that we optimise the desktop
>and we have done that (in fact we do that every month) to no avail.
>
>        Please advise.





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