In article <5gk2in$qta at net.bio.net>, David Needleman,
davidn at utmmg.med.uth.tmc.edu wrote:
> We have installed an XL upgrade on our 377. We typically load either 36
> or 48 lanes and have had no problems loading either configuration.
>> Unfortunately, I cant say the same for the tracking. As long as every
> lane contains data, the tracking problems are usually minimal. However,
> if one or more lanes has no sequence data or very little data, tracking
> can become a nightmare. The tracking lines are there, but they are
> evenly spaced and have not tracked a single bit of data. We either have
> to manually retrack all the lanes or go back to the sample sheet,
> determine which lanes have no data, tell the computer that these lanes
> contain no sample, autotrack and hope for the best.
>> Good luck,
>> David
> Director, Molecular Genetics Core Facility
> Microbiology and Molecular Genetics
> UT-Houston Medical School
We have been running the XL upgrade on our 373 for a 4-5 months now and
have had few problems and are using 64 lanes almost all the time. Loading
takes a while but is not a problem.
I agree that sometime the tracking can fail if a few lanes are blank. We
are able to resolve this every time by de-selecting the blank samples on
the sample sheet, and the re-tracking the gel. Usually a few samples need
some manual tracking modifications, but this takes a few minutes.
Good luck,
Alan.
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