Blue blobs on ABI 377

Valkyrie Valkyrie
Wed Mar 5 09:01:49 EST 1997

Anybody ever experience a big blue blob that appears during a sequence run
on an ABI 377 using 4% acrylamide?  It will typically appear after the run
has started and will mask between 5 and 30 out of 36 reads!!  It's 
a problem with gel composition but I am unsure exactly what could be
affecting the mix.  It occurs with about 1 in 20 gels.  Any suggestions to
fix the problem are welcome.  

tmajor at utdallas.edu

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