yac sequencing

Rashmi Rashmi
Wed Jul 9 08:20:28 EST 1997

Does anyone have any experience sequencing Yacs directly? We have been 
sequencing P1s and Bacs sucessfully but the same protocol is not 
working for Yacs. Any information regarding the method of DNA 
preparation and cycling condtions would be greatly appreciated. we use 
ABI sequencers.

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