Dear Sequencers,
we have been seeing stretching of peaks from around 190
to 210 bp across the gel on a 377 with resulting loss of resolution. The
peaks return to their normal width after this so it seems to be a
localised problem at around these fragment sizes. We are doing 3.5 hour
runs on 36cm plates using dye-terminator chemistry with the ABI kit on
double stranded plasmid DNA. Has anyone seen this and know what might be
causing it?
Thanks in advance
Tim Sawbridge
T.Sawbridge at forbio.com.au
