I am making my second attempt at using LR at the 4x speed on the 377. I
would like to know how the gels are being made up. LR, TBE, urea, water
Temed and APS. Are you degassing and if so for how long. My first gel
made by the FMC recipe was not very good beyond 450 bases compared to my
cheap, cheap 29:1 acrylamide. We use an Otter to pour the gels.
Hal
Harold G. Hills, Ph.D. DNA Sequencing Specialist 515 294-9585
1184 Molecular Biology Building FAX 515 294-1597
Iowa State University
Ames, IA 50011-3260 hhills at iastate.edu
