I have been having difficulty with the tightness of 36 well shark's
tooth combs on the 377. I am using ABI 36 cm. plates with Long Ranger
gel or Sooner 4.25% gel. I pour the plates nearly flat. The comb is
sometimes so loose that I have dislodged it rinsing the wells, and
sometimes so tight that I break teeth putting it in. I have tried
calibrating the angle at which I pour the plates and using tight or
loose clamps for the well. Never-the-less, I am unable to control the
tightness. I suspect that the plates deform gradually in some way.
Has anyone had/solved this problem?
--
Tom Kotlar
Northwestern University
t-kotlar at nwu.edu
