Dear Friends,
We have been using Long Ranger gel solution from FMC on our 373 (~5.0%)
and 377 (5.5%) machines but we have not been able to get long reads, as
claimed in the advertisements. I am requesting others who are getting
longer reads to please share their sequencing secrets with us.
I wanted to get some idea before playing around with gels
and running conditions. Especially if someone has compared GENE-PAGE Plus
and Long Ranger because some of the genome centers are using PAGE PLUS.
Your comments will be very valuable for us.
Cheers
Muhammad A. Lodhi
Cold Spring Harbor Lab.