Sequencing G-C Rich plasmids

Wed Feb 26 14:52:38 EST 1997

	Dear all,

										does anyone have any suggestions for sequencing G-C rich 
plasmids ? : We have tried all the usual tricks, including doubling the 
amount of taq & primer ( sequencing is based on diterminator chemistry, 
utilising an ABI 377 ), increasing the denaturation temp. and the 
denaturation time and finally denaturing the plasmid itself prior to 
cycle sequencing. Is there anything left to try ?



																																				Laurence S. Hall.
Laurence Hall,
School of Biological Sciences,
2.237 - Biomolecules,
Division of Biochemistry,
Stopford Building,
University of Manchester,
Oxford Rd.
Manchester M13 9PT.

Tel. : +44 (0)161 275 6916.                E-mail : 
LHALL at fs2.scg.man.ac.uk 
Fax		: +44 (0)161 5082.	

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