Sequencing through poly A region

Juergen Juergen
Wed Feb 19 16:44:48 EST 1997

In article <5ct2rc$f64 at net.bio.net>, Muhammad A. Lodhi, lodhi at cshl.org 

> experience of sequencing
> through a region of ~22 As

get out the good old DMS, hydrazine, piperidine etc. and sequence it the
Maxam-Gilbert way.  I had no trouble to sequence through a stretch of 22
A-residues in the rat BC1 RNA genomic sequence.  PCR is often the cause of
trouble, since the polymerase stutters through homopolymers.

Good luck,

                                          usual disclaimers
Juergen Brosius
Inst. f. eXp. Pathol.
University of Muenster
Von-Esmarch-Str. 56
D-48149 Muenster, Germany

Fax: +49  251  835 8512
Tel: +49  251  835 8511

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