I had a similar problem with another Invitrogen vector pRSET. It turned
out that the vector contained a 'non-standard' T7 primer sequence which
differed from the usual T7-primer at the 3'end. After I rechecked the
actual pRSET T7 sequence and synthesized the appropriate T7 primer
sequencing went very well. Thus, you may want to have the investigator
check the actual PCRBlunt vector primer sequences from Invitrogen.
Hope this helps,
Brad Turner
**************************************************************
Bradley Turner
Beth Israel Deaconess
Medical Center
Harvard Medical School 617-667-1215 phone
Division of Gastroenterology 617-667-2767 fax
Room Dana 536 bsturner at mbcrr.harvard.edu
330 Brookline Avenue bturner at bidmc.harvard.edu
Boston, MA 02215, USA turner at sprcore.bidmc.harvard.edu
**************************************************************
BEGIN INCLUDED
MESSAGE------------------------------------------------------
