Alexander Kraev kraev at bc.biol.ethz.ch
Thu Dec 4 14:50:32 EST 1997

Dear base busters,
A person at a perkin Elmer seminar here mentioned that he used a thermostable
topoisomerase "fidelase" to directly sequence bacterial chromosomal DNA using
BigDye terminator kit. Could anyone else please share their experience
with this enzyme?


Alexander Kraev, PhD

Alexander Kraev, PhD
Biochemie III, ETHZ Zurich
Phone 41-1-632-31-47
Fax 41-1-632-12-13
e-mail kraev at bc.biol.ethz.ch

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