More fade out problems

Alison Reavley alison.reavley at zoology.oxford.ac.uk
Mon Dec 1 08:34:49 EST 1997

Thanks for the reply, the bands are fading into the background, not dis
appearing abruptly, this occurs in every lane.   Its an interesting
point though about the laser overheating and I'll check it out.  Thanks
for the advice
Alison Reavley

 On 29 Nov 1997, Tony Major wrote:

> On that second problem, is it actually fading or abruptly disappearing? 
> Because we had two of our 377's that were not properly ventilated and
> when the laser overheated, the sequence completely blacked out and when
> the laser cooled off, it came back on as if 'magically reappearing.'  Is
> it every lane?
> -Tony Major
> Genome Science and Technology Center
> UT Southwestern Med. Center
> Dallas, TX
> Alison Reavley wrote:
> > 
> > Hi,
> > I have followed with interest the problems people have been having with
> > 373 and 377 gels, concerning them fading out after a cetrain number of
> > base pairs.  Our lab has had the same problems with 377 gels and is trying
> > to solve it by acid/alkali washes.  We have now come across another
> > problem where our bands fade out in the middle section of the gel after
> > about 200 bases then these reappear as if by magic.  Has anyone else seen
> > this and maybe already solved it?
> > Thanks in advance
> > Alison Reavley

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