Dear Sander Peters, Hans Sandbrink and Newsgroupers,
I have been using Sequencher to assemble a geneome sized project which
currently contains 2800+ sequences . The Mac that I use is a 7600/132
with 80 Meg RAM built in and 60 Meg vitrual RAM , I would recommend
getting the most RAM that you can afford , one of our machines has 150
Meg which handles anything that is thrown at it . I would also highly
recomend a Jaz drive for the data storage as the projects can get very
large .
I have reached the stage where if all the individual reads are taken out
of contigs they can not be viewed within the Sequencher project window
but that is also true when they are exported to a Mac folder . All the
functions still work to specifications you just need to have a little
faith that the select all command does in fact select all the reads .
I have found that the excelent graphic user interface for contig editing
has made a mamoth job a lot easier and I feel more confident being able
to edit with the chromatogram data directly below the text alignment .
Sequencher may not have been designed for this type of work but it copes
admirably and my hat is again off to the people at Gene Codes , may they
keep up the good work .
Yours Sincerely ,
David Cain
Administrator
Biosci.genome.autosequencing
Larkseq at easervices.com
