IUBio

GeneScan Analysis of Microsattelites

M. M.
Fri Aug 8 08:53:22 EST 1997


Hi!

I am having a bit of a problem with GeneScan analysis.  I am studying
different microsattelites using the Research Genetics Weber Screening
Set Ver 6.  If for a particular Microsat I am suppossed to get 186,
190bp (and so on) bands, I get bands which are sized as 188 using TAMRA
350 or 500 internal lane standard, now how am I suppossed to size these
bands, should I classify the band as 186bp or 190bp?  If anyone has a
similar problem and knows how to resolve it kindly email me.  Thanks for
your help.

Dr. Raheel Qamar




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