We are having a problem with our 373 upgrade sequencer. The PMT value
must be set at around 800 to get a blue line (y value) of around 700. The
laser and filter were just replaced (ABI thought that this was the
problem). The machine was just inherited from a lab that shut down so we
do not know the history. The standardish PMT setting on other machines is
usually around 530-560. Has anyone had this problem in the past and know
what should be done to correct it? I have heard of these high settings
when using the base-sprinter but never when doing a normal overnight run.
Thanks in advance for any help.
Mike
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Michael C. Gorry
University of Pittsburgh
Center for Genomic Sciences
mcgorry at med.pitt.edu
