Skipped region.

Peter Peter
Thu Apr 10 16:12:39 EST 1997

Yes, I have seen this happen with a clone which contained a large inverted
repeat.  The sequence read through the vector sequence to the cloning 
skipped over the inverted repeat and continued on the other side.  

Peter J. Myler, Ph.D.
Staff Scientist	                                Assistant Professor
Seattle Biomedical Research Institute	Department of Pathobiology
4 Nickerson Street	                                University of 
Seattle, WA  98109-1651	                Seattle, WA  98195
e-mail:	mylerpj at sbri.org	                mylerpj at u.washington.edu
Phone:	(206)-284-8846x332
FAX:	(206)-284-0313

charlie demma; demma at welchlink.welch.jhu.edu wrote in article
<5ibmdq$pt at net.bio.net>...
> We did some sequencing for someone on an ABI 377. He was absolutely
> positive that his insert was in his plasmid but the insert was missing
> from his electropherogram. Has anyone ever seen a situation where the
> polymerase will skip a region and continue on to give a nice sequence? 
> Charlie Demma
> Demma at welchlink.welch.jhu.edu

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