Hello fellow sequencers,
I am beginning a project in which I will be sequencing a few thousand
PCR products using an 377 DNA sequencer. Because of the number of PCR
products, I would like to use dye-terminator chemistries and a 96-well
format. I am a little concerned about obtaining a "clean" (no primers,
salts, etc.) PCR product so that I can use the dye-terminator
chemistries without getting interference from the PCR primers and rxn
mix. I was wondering if anyone has some good ideas on how to clean the
PCRs in a 96-well format.(or should I resort to dye-primer chemistries)
Thank You in advance,
Larry Grocki
Lexicon Genetics
<lgrocki at lexgen.com>
