We use 10 microlitre reactions on a 373A with Stretch and 36 lane loading.
The reads are usually almost as good as 20 microlitres. No special
tricks,
just half of everything.
Geoff McFadden
Geoffrey Ian McFadden PhD
Senior Lecturer phone: +61 3 9457 5772 (home)
Plant Cell Biology Research Centre phone: +61 3 9344 5053 (lab)
School of Botany phone: +61 3 9344 5054 (office)
University of Melbourne fax: +61 3 9347 1071
Parkville VIC 3052
http://128.250.102.45/McFaddenLab.html
AUSTRALIA email: mc_fadden at botany.unimeLb.edu.au
