Dear Sequenchers ,
Just to add something to the software debate , I have been using Sequenchers
( from Gene Codes : www.genecodes.com ) for a number of months.
On both small ( single primer extensions ) and large scale projects ( Cosmid
size by shotgun methodology ) it is extremely user friendly and packed with
useful features . In fact, Sequencher covers a number of applications such
as automatic vector trimming that I had previously used seperate programs for
( programs which, I might add, cost as much as Sequencher despite not doing
the whole job) .
Being able to edit the data without effecting the original base calls make
cleaning up of contig consensus sequence much easier . Restriction mapping
tools and clear contig overview maps are easy to get to and intelligently
integrated . I'm not one to endorse specific products , but I've used
several different sequencing analysis programs , and the combination of
automated trimming , fast fragment assembly , and great editing tools make
Sequencher the hands-down favorite in our lab .
I hope that this helps some users and that Sequencher continues to develop in
the right direction .
Yours Sequencingly ,
David Cain
P.S. I was totally amazed the other day when a few of my collegues started
writting thier own Apple scripts to work with Sequencher . It is a big bonus
being able to add extra features and tailor it to your specific needs .
